step

Full identifier: http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step

Assigned to 1 class:

Minted in Nanopublication

 RAu8IsZZpT Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mycobacteria" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mycobacteria" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#_3 _3 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "culture medium" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#_4 _4 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "TE" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#step step http://purl.org/net/SMARTprotocol#hasExperimentalInput hasExperimentalInput This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#_1 _1 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#step step https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#_2 _2 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#_3 _3 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#_4 _4 .
This is the identifier for this whole nanopublication. http://purl.org/np/RAu8IsZZpT... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#step step .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAu8IsZZpT... this nanopublication .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAu8IsZZpT...#sig sig http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal) "RSA" .

Nanotate, 10 Mar 2021, 11:20:30 UTC

References

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T14:44:11.546Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T14:44:11.546Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T14:44:11.546Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T11:20:30.406Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette. Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.
Nanotate
2021-03-10T11:20:30.406Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T11:20:30.406Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T11:20:30.406Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T11:20:30.406Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T11:20:30.406Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T11:20:30.406Z

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