step

Full identifier: http://purl.org/np/RAsJyZ5Znc54-tK37PmFveFPCA0USROZ7tO4XVMsraHW4#step

Assigned to 1 class:

Minted in Nanopublication

 RAsJyZ5Znc Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "cells" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "cells" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#step step http://purl.org/net/SMARTprotocol#hasExperimentalOutput hasExperimentalOutput This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#_1 _1 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#step step https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#_2 _2 .
This is the identifier for this whole nanopublication. http://purl.org/np/RAsJyZ5Znc... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#step step .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAsJyZ5Znc... this nanopublication .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAsJyZ5Znc...#sig sig http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal) "RSA" .

Nanotate, 5 Mar 2021, 10:08:35 UTC

References

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:08:35.231Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice. Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used.
Nanotate
2021-03-05T10:08:35.231Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:08:35.231Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:08:35.231Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:08:35.231Z
links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo
step
Nanotate
2021-03-05T10:08:35.231Z

Raw

TriG(txt), JSON-LD(txt), N-Quads(txt), XML(txt)