step
Full identifier: http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
Assigned to 1 class:
Minted in Nanopublication
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#_1
_1
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"pellet"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#_2
_2
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"tubes"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#_3
_3
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"TE"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
http://purl.org/dc/elements/1.1/description
description
(this is a literal)
"After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)."
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
http://purl.org/net/SMARTprotocol#hasExperimentalOutput
hasExperimentalOutput
http://purl.obolibrary.org/obo/CHEBI_16991
CHEBI_16991
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
type
http://purl.org/net/p-plan#Step
Step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
http://purl.obolibrary.org/obo/CHEBI_16991
CHEBI_16991
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#_1
_1
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#_2
_2
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218
C62218
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#_3
_3
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
The assertion above
http://purl.org/pav/createdWith
createdWith
https://nanotate.bitsfetch.com
nanotate.bitsfetch.com
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
The assertion above
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-02-25T16:19:32.955991"
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
The assertion above
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
zuluaga.lizzz
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAqSdY5sD_...
This nanopublication
the nanopublication consists of an introduction of the given resource
http://purl.org/nanopub/x/introduces
introduces
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#step
step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#sig
sig
http://purl.org/nanopub/x/hasSignatureTarget
has as target
This is the identifier for this whole nanopublication.
http://purl.org/np/RAqSdY5sD_...
this nanopublication
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#sig
sig
http://purl.org/nanopub/x/hasAlgorithm
has the algorithm
(this is a literal)
"RSA"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#sig
sig
http://purl.org/nanopub/x/hasPublicKey
has the public key
(this is a literal)
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAqSdY5sD_...#sig
sig
http://purl.org/nanopub/x/hasSignature
has the signature
(this is a literal)
"CK0mUuEnSryqDlktLiDzRtTbtqvviyX1sWeq4FLSkqK62YSYfkFnQ/HwTyKezY7DqmGU2q8fUJx2xP1NI/uAPsPyWpMoNuOkKce6F1rlz3waaMHVCHWflyKmCQyXKibwDd0VS/NSBecXujFnqAuLY0mDrXc4VtLm5xIcKrh8YTk="
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/0vwt1neuEeugLneRnV11IQ
0vwt1neuEeugLneRnV11IQ
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/U5V5WHeuEeuaqaeDOnia0g
U5V5WHeuEeuaqaeDOnia0g
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/Zutt3HeuEeuuZH8J3g6HLA
Zutt3HeuEeuuZH8J3g6HLA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/i38vTneuEeuNAsMsRIF24w
i38vTneuEeuNAsMsRIF24w
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/oNTjoneuEeu3I3Om5Ihl4A
oNTjoneuEeu3I3Om5Ihl4A
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAqSdY5sD_...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/uGfetneuEeuQLE_Q4_h28w
uGfetneuEeuQLE_Q4_h28w
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAqSdY5sD_...
this nanopublication
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-02-25T16:19:32.955991"
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAqSdY5sD_...
this nanopublication
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
zuluaga.lizzz
.
References
| Nanopublication | Part | Subject | Predicate | Object | Published By | Published On |
|---|---|---|---|---|---|---|
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:32:30.020Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:32:30.020Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:19:32.955Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0).
|
Nanotate
|
2021-02-25T16:19:32.955Z
|
||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:19:32.955Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:19:32.955Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:19:32.955Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:19:32.955Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:19:32.955Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-25T16:19:32.955Z
|