step

Full identifier: http://purl.org/np/RAZQSfTf7hdqYE1qFLykuQB4o3Oftn9vZTlXwsJpPLiCM#step

Assigned to 1 class:

Minted in Nanopublication

 RAZQSfTf7h Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "cell suspension" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "cell suspension" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#_3 _3 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "24 well culture plate" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#step step http://purl.org/net/SMARTprotocol#hasExperimentalOutput hasExperimentalOutput This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#_1 _1 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#step step https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#_2 _2 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#step step https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#_3 _3 .
This is the identifier for this whole nanopublication. http://purl.org/np/RAZQSfTf7h... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#step step .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAZQSfTf7h... this nanopublication .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAZQSfTf7h...#sig sig http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal) "RSA" .

Nanotate, 5 Mar 2021, 10:03:51 UTC

References

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates.
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo
step
Nanotate
2021-03-05T10:03:51.750Z

Raw

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