step
Full identifier: http://purl.org/np/RAYDwTtT3SWmPi5y6QPlgud6dumB_mJt6XQ3oYluNNiVU#step
Assigned to 1 class:
Minted in Nanopublication
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_1
_1
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"mosquitoes"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_2
_2
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"mosquitoes"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_3
_3
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"micropistle"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_4
_4
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"Rnase A"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_5
_5
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"Proteinase K"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_6
_6
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"1X STE buffer "
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
http://purl.org/dc/elements/1.1/description
description
(this is a literal)
"Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it."
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
hasExperimentalInput
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_1
_1
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
type
http://purl.org/net/p-plan#Step
Step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_2
_2
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_3
_3
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
http://www.bioassayontology.org/bao#BAO_0010014
BAO_0010014
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
http://purl.obolibrary.org/obo/CHEBI_17992
CHEBI_17992
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
http://purl.obolibrary.org/obo/CHEBI_29234
CHEBI_29234
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
http://purl.obolibrary.org/obo/CHEBI_8984
CHEBI_8984
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_4
_4
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_5
_5
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#_6
_6
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
The assertion above
http://purl.org/pav/createdWith
createdWith
https://nanotate.bitsfetch.com
nanotate.bitsfetch.com
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
The assertion above
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-02-24T15:57:29.331725"
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
The assertion above
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
zuluaga.lizzz
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAYDwTtT3S...
This nanopublication
the nanopublication consists of an introduction of the given resource
http://purl.org/nanopub/x/introduces
introduces
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#step
step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#sig
sig
http://purl.org/nanopub/x/hasSignatureTarget
has as target
This is the identifier for this whole nanopublication.
http://purl.org/np/RAYDwTtT3S...
this nanopublication
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#sig
sig
http://purl.org/nanopub/x/hasAlgorithm
has the algorithm
(this is a literal)
"RSA"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#sig
sig
http://purl.org/nanopub/x/hasPublicKey
has the public key
(this is a literal)
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAYDwTtT3S...#sig
sig
http://purl.org/nanopub/x/hasSignature
has the signature
(this is a literal)
"aWhdnmGhJ66HdQT7ErQofYYVZALQSx0vSYV2bxZ9SyR8RnJ6zgt68Y6GIWNMMc6hLBRV5YxFXMEs4JA8KehE8EK+QlYjhEiP1q5Km6xmWcFngNWmrVfg2HQbw7jgUlZ6licoQwCN1GmXIv57ElrxNKIP3ghmVtOCEqodFcpRE8E="
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/1x5l6HbfEeuNLNOMl31uWw
1x5l6HbfEeuNLNOMl31uWw
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/AMqrLHbgEeu7t0tnnnh5sA
AMqrLHbgEeu7t0tnnnh5sA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/JtjBvnbgEeu9UyOKVQTWOQ
JtjBvnbgEeu9UyOKVQTWOQ
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/RU7bGnbgEeudMq9-HaiY6A
RU7bGnbgEeudMq9-HaiY6A
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/V0kdJnbgEeurc_usb1nrtA
V0kdJnbgEeurc_usb1nrtA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/aWt3LnbgEeuUGLMXogyyYg
aWt3LnbgEeuUGLMXogyyYg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/e-8U2HbfEeuds-e26QqsLg
e-8U2HbfEeuds-e26QqsLg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/juDxOHbfEeuuK9Oh6-VO3w
juDxOHbfEeuuK9Oh6-VO3w
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/ql2CZHbfEeuFGXO0qGYX7g
ql2CZHbfEeuFGXO0qGYX7g
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAYDwTtT3S...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/vYrwknbfEeuT7hPT-uOLOQ
vYrwknbfEeuT7hPT-uOLOQ
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAYDwTtT3S...
this nanopublication
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-02-24T15:57:29.331725"
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAYDwTtT3S...
this nanopublication
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
zuluaga.lizzz
.
References
| Nanopublication | Part | Subject | Predicate | Object | Published By | Published On |
|---|---|---|---|---|---|---|
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T16:10:21.737Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T16:10:21.737Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T15:57:29.331Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it.
|
Nanotate
|
2021-02-24T15:57:29.331Z
|
||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T15:57:29.331Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T15:57:29.331Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T15:57:29.331Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T15:57:29.331Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T15:57:29.331Z
|
|||
|
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-02-24T15:57:29.331Z
|