step
Full identifier: http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step
Searching for classes... 
Searching for instances...
Searching for templates...
Minted in Nanopublication
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_1
_1
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"mosquitoes"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_10
_10
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"1% SDS"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_2
_2
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"1% Triton -X"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_3
_3
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"mosquitoes"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_4
_4
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"micropistle"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_5
_5
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"1.5 ml eppendorf tube"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_6
_6
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"1X STE buffer"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_7
_7
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"Proteinase K (20mg/ml)"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_8
_8
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"100mM sucrose"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_9
_9
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"Rnase A (20mg/ml)"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
http://purl.org/dc/elements/1.1/description
description
(this is a literal)
"Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it."
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
hasExperimentalInput
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_1
_1
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
type
http://purl.org/net/p-plan#Step
Step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_3
_3
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_4
_4
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_5
_5
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_10
_10
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_2
_2
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_6
_6
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_7
_7
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_8
_8
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#_9
_9
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
The assertion above
http://purl.org/pav/createdWith
createdWith
https://nanotate.bitsfetch.com
nanotate.bitsfetch.com
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
The assertion above
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-03-10T10:33:19.355370"
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
The assertion above
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/alejandra.sandoval
alejandra.sandoval
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAL4fGVNEl...
This nanopublication
the nanopublication consists of an introduction of the given resource
http://purl.org/nanopub/x/introduces
introduces
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#step
step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#sig
sig
http://purl.org/nanopub/x/hasSignatureTarget
has as target
This is the identifier for this whole nanopublication.
http://purl.org/np/RAL4fGVNEl...
this nanopublication
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#sig
sig
http://purl.org/nanopub/x/hasAlgorithm
has the algorithm
(this is a literal)
"RSA"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#sig
sig
http://purl.org/nanopub/x/hasPublicKey
has the public key
(this is a literal)
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAL4fGVNEl...#sig
sig
http://purl.org/nanopub/x/hasSignature
has the signature
(this is a literal)
"Kd4Aa6fDdpMWhH77AgLNFo/GE7w50Y0bXMfwIOIVdP+2Jp7DcBZ3K28i9HOzqkwNBC9YZmAKwLlwHp818FZw1VIUbdMpPIaCSwdAsSJgsv/Ov/vuO6093NjHOignVj4QsE9+XO9WZOyFBX1XxDAbO2anaVMuoEQa4HYFefmlDOc="
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/8b4bAHJNEeu01RN1eV0q-Q
8b4bAHJNEeu01RN1eV0q-Q
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/ESqevnJOEeu1UX9lnqErIQ
ESqevnJOEeu1UX9lnqErIQ
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/IrIzfHJOEeujx7t3IvpXzA
IrIzfHJOEeujx7t3IvpXzA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/OkmgznJOEeudNEPmE26k-Q
OkmgznJOEeudNEPmE26k-Q
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/UBFH9HJOEeusy1eAko5oQw
UBFH9HJOEeusy1eAko5oQw
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/Usa7nHJNEeuoHBdiYKYtlg
Usa7nHJNEeuoHBdiYKYtlg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/cWBYjnJMEeuOKD-DjOC3Cw
cWBYjnJMEeuOKD-DjOC3Cw
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/drQGkHJNEeudQGs6yWujow
drQGkHJNEeudQGs6yWujow
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/lG67TnJNEeu01GMwoWiMRA
lG67TnJNEeu01GMwoWiMRA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAL4fGVNEl...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/rawtGHJMEeu0J4cNJ9O11g
rawtGHJMEeu0J4cNJ9O11g
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAL4fGVNEl...
this nanopublication
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-03-10T10:33:19.355370"
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAL4fGVNEl...
this nanopublication
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/alejandra.sandoval
alejandra.sandoval
.
References