_6
Full identifier: http://purl.org/np/RAKpSHKnh8UHKZczlcfbZdI4kJgcAx7985zW8gRt3MOtM#_6
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Minted in Nanopublication
http://purl.obolibrary.org/obo/PO_0025034
PO_0025034
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"leaf"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_1
_1
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"recalcitrant samples"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_2
_2
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"recalcitrant samples"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_3
_3
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"mortar"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_4
_4
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"pestle"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_5
_5
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"extraction buffer"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_6
_6
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"liquid nitrogen"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
http://purl.org/dc/elements/1.1/description
description
(this is a literal)
"Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
hasExperimentalInput
http://purl.obolibrary.org/obo/NCBITaxon_1
NCBITaxon_1
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
hasExperimentalInput
http://purl.obolibrary.org/obo/PO_0025034
PO_0025034
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
hasExperimentalInput
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_1
_1
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
hasExperimentalInput
http://www.ebi.ac.uk/efo/EFO_0000992
EFO_0000992
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
type
http://purl.org/net/p-plan#Step
Step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
http://purl.obolibrary.org/obo/NCBITaxon_1
NCBITaxon_1
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
http://purl.obolibrary.org/obo/PO_0025034
PO_0025034
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_2
_2
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
http://www.ebi.ac.uk/efo/EFO_0000992
EFO_0000992
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_3
_3
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_4
_4
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_5
_5
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#_6
_6
.
http://www.ebi.ac.uk/efo/EFO_0000992
EFO_0000992
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"shoot"
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
The assertion above
http://purl.org/pav/createdWith
createdWith
https://nanotate.bitsfetch.com
nanotate.bitsfetch.com
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
The assertion above
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-03-10T10:13:55.313885"
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
The assertion above
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/alejandra.sandoval
alejandra.sandoval
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAKpSHKnh8...
This nanopublication
the nanopublication consists of an introduction of the given resource
http://purl.org/nanopub/x/introduces
introduces
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#step
step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#sig
sig
http://purl.org/nanopub/x/hasSignatureTarget
has as target
This is the identifier for this whole nanopublication.
http://purl.org/np/RAKpSHKnh8...
this nanopublication
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#sig
sig
http://purl.org/nanopub/x/hasAlgorithm
has the algorithm
(this is a literal)
"RSA"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#sig
sig
http://purl.org/nanopub/x/hasPublicKey
has the public key
(this is a literal)
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAKpSHKnh8...#sig
sig
http://purl.org/nanopub/x/hasSignature
has the signature
(this is a literal)
"YMZ4XWZSNv2IF7Nwx7TIa2W+G+wqh1zbsvy8wFq1arAhcaU09B05T5vfvy2LO1xRZh0XwYBy28VwIwl2hJq3fOboDgtotvODg2GWDcnVMxfjqJc/1uib/E8o+TyC75xYRQNkyMSrhqgZzS977Qk9ZPzkv+8Pac0YHLfyJQYU59M="
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/85nfmHJFEeupsZf00tQnZA
85nfmHJFEeupsZf00tQnZA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/GR0bVHJGEeuiqkcaESmO6Q
GR0bVHJGEeuiqkcaESmO6Q
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/OWfHdHJGEeujmAvPIUDH6w
OWfHdHJGEeujmAvPIUDH6w
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/USf8vHJGEeub4tMCkIYnUA
USf8vHJGEeub4tMCkIYnUA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/c0g59nJGEeuhuXe3r_IOtA
c0g59nJGEeuhuXe3r_IOtA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/h_gQsHJGEeujy2Ps728ADg
h_gQsHJGEeujy2Ps728ADg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/owhuNnJGEeuFPWP_py-RsA
owhuNnJGEeuFPWP_py-RsA
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/rbSDonJFEeuo0j902ftpgg
rbSDonJFEeuo0j902ftpgg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAKpSHKnh8...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/teA4hnJGEeuqgNcqv6DR-w
teA4hnJGEeuqgNcqv6DR-w
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAKpSHKnh8...
this nanopublication
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-03-10T10:13:55.313885"
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAKpSHKnh8...
this nanopublication
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/alejandra.sandoval
alejandra.sandoval
.
References