step

Full identifier: http://purl.org/np/RAHi9L2Ftk2pQ_lSeWbu23B7opN3IajkEAm8CAs35q7eE#step

Minted in Nanopublication

 RAHi9L2Ftk Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RAHi9L2Ftk...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "100 mg/ml lysozyme solution" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAHi9L2Ftk...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAHi9L2Ftk...#_1 _1 .
This is the identifier for this whole nanopublication. http://purl.org/np/RAHi9L2Ftk... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAHi9L2Ftk...#step step .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAHi9L2Ftk...#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAHi9L2Ftk... this nanopublication .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAHi9L2Ftk...#sig sig http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal) "RSA" .

Nanotate, 20 Mar 2021, 05:39:47 UTC

References

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:37:05.476Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:37:05.476Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:37:05.476Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:39:47.164Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath. Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.
Nanotate
2021-03-20T05:39:47.164Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:39:47.164Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:39:47.164Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:39:47.164Z
links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo
step
Nanotate
2021-03-20T05:39:47.164Z

Raw

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