step

Full identifier: http://purl.org/np/RA6F1VxP7thp4zX-1raNBn2QGqYfK0d8XZO_-jUUWiqNE#step

Assigned to 1 class:

Minted in Nanopublication

 RA6F1VxP7t Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mycobacteria" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mycobacteria" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#_3 _3 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "TE" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#step step http://purl.org/net/SMARTprotocol#hasExperimentalInput hasExperimentalInput This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#_1 _1 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#step step https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#_2 _2 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#_3 _3 .
This is the identifier for this whole nanopublication. http://purl.org/np/RA6F1VxP7t... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#step step .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RA6F1VxP7t... this nanopublication .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA6F1VxP7t...#sig sig http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal) "RSA" .

Nanotate, 22 Mar 2021, 16:05:00 UTC

References

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-22T16:05:00.244Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette. Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.
Nanotate
2021-03-22T16:05:00.244Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-22T16:05:00.244Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-22T16:05:00.244Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-22T16:05:00.244Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-22T16:05:00.244Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-22T16:05:00.244Z
links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo
step
Nanotate
2021-03-22T16:05:00.244Z

Raw

TriG(txt), JSON-LD(txt), N-Quads(txt), XML(txt)