step

Full identifier: http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step

Assigned to 1 class:

Minted in Nanopublication

 RA3MmCHHYD Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mosquitoes" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mosquitoes" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_3 _3 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "micropistle" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_4 _4 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "Rnase A (20mg/ml)" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_5 _5 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1X STE buffer" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_6 _6 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "Proteinase K (20mg/ml)" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step step http://purl.org/net/SMARTprotocol#hasExperimentalInput hasExperimentalInput This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_1 _1 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step step https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_2 _2 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step step https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_3 _3 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_4 _4 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_5 _5 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_6 _6 .
This is the identifier for this whole nanopublication. http://purl.org/np/RA3MmCHHYD... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step step .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RA3MmCHHYD... this nanopublication .
This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#sig sig http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal) "RSA" .

Nanotate, 16 Mar 2021, 16:48:04 UTC

References

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:17:56.166Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:17:56.166Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-16T16:48:04.108Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it.
Nanotate
2021-03-16T16:48:04.108Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-16T16:48:04.108Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-16T16:48:04.108Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-16T16:48:04.108Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-16T16:48:04.108Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-16T16:48:04.108Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-16T16:48:04.108Z

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