Nanopublication: RA3MmCHHYD
Full identifier: http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_1
_1
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"mosquitoes"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_2
_2
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"mosquitoes"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_3
_3
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"micropistle"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_4
_4
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"Rnase A (20mg/ml)"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_5
_5
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"1X STE buffer"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_6
_6
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"Proteinase K (20mg/ml)"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
http://purl.org/dc/elements/1.1/description
description
(this is a literal)
"Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it."
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
hasExperimentalInput
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_1
_1
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
type
http://purl.org/net/p-plan#Step
Step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_2
_2
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_3
_3
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
http://www.bioassayontology.org/bao#BAO_0010014
BAO_0010014
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
http://purl.obolibrary.org/obo/CHEBI_17992
CHEBI_17992
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
http://purl.obolibrary.org/obo/CHEBI_29234
CHEBI_29234
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
http://purl.obolibrary.org/obo/CHEBI_8984
CHEBI_8984
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_4
_4
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_5
_5
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#_6
_6
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
The assertion above
http://purl.org/pav/createdWith
createdWith
https://nanotate.bitsfetch.com
nanotate.bitsfetch.com
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
The assertion above
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-03-16T16:48:04.108013"
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
The assertion above
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/iannotate
iannotate
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RA3MmCHHYD...
This nanopublication
the nanopublication consists of an introduction of the given resource
http://purl.org/nanopub/x/introduces
introduces
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#step
step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#sig
sig
http://purl.org/nanopub/x/hasSignatureTarget
has as target
This is the identifier for this whole nanopublication.
http://purl.org/np/RA3MmCHHYD...
this nanopublication
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#sig
sig
http://purl.org/nanopub/x/hasAlgorithm
has the algorithm
(this is a literal)
"RSA"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#sig
sig
http://purl.org/nanopub/x/hasPublicKey
has the public key
(this is a literal)
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA3MmCHHYD...#sig
sig
http://purl.org/nanopub/x/hasSignature
has the signature
(this is a literal)
"aZd/AL+wAll/02gNVAmhv8QGPBCTs7otfD4kaMz6CzndlDewoaVZcCmCBsl0xltZZZisvKm9bu+mhYyIwYX+5z4L3rxcyLp7AxPMD7roKQ7W9/5gRzY5DSAJH9kE9Y6jZKoTkDGwyXty1qOddP/83ut8S3q0w1HrYrcGVrpP6OU="
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/8Y0HtIJ9EeuxMKftfvVUrw
8Y0HtIJ9EeuxMKftfvVUrw
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/AHbk6IJ-EeuLn7c9eitXuQ
AHbk6IJ-EeuLn7c9eitXuQ
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/CWoxtIahEeueMafFr2xIRQ
CWoxtIahEeueMafFr2xIRQ
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/FBJa-oJ-EeuVON8E0bPEnQ
FBJa-oJ-EeuVON8E0bPEnQ
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/GGaOiIahEeuEzbud0kuutg
GGaOiIahEeuEzbud0kuutg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/JzPoLoJ-EeubeTMBRz-oHg
JzPoLoJ-EeubeTMBRz-oHg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/P9yYHIJ-Eeuk1J8X-v5LOg
P9yYHIJ-Eeuk1J8X-v5LOg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/VhkMtIJ-EeuaQsuiGJkaPw
VhkMtIJ-EeuaQsuiGJkaPw
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/dCvMjIJ-EeuYqLMMrOutfQ
dCvMjIJ-EeuYqLMMrOutfQ
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA3MmCHHYD...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/i-hYaIJ-EeuaRdNsg6w5Hg
i-hYaIJ-EeuaRdNsg6w5Hg
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RA3MmCHHYD...
this nanopublication
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-03-16T16:48:04.108013"
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RA3MmCHHYD...
this nanopublication
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/iannotate
iannotate
.