References for: step
Full identifier: http://purl.org/np/RAsJyZ5Znc54-tK37PmFveFPCA0USROZ7tO4XVMsraHW4#step
Nanopublication | Part | Subject | Predicate | Object | Published By | Published On |
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
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step
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Nanotate
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2021-03-05T10:18:38.096Z
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
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step
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Nanotate
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2021-03-05T10:18:38.096Z
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
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step
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Nanotate
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2021-03-05T10:18:38.096Z
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
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step
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Nanotate
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2021-03-05T10:08:35.231Z
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
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step
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Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice.
Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used.
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Nanotate
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2021-03-05T10:08:35.231Z
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-03-05T10:08:35.231Z
|
|||
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-03-05T10:08:35.231Z
|
|||
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-03-05T10:08:35.231Z
|
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links a nanopublication to its pubinfo
http://www.nanopub.org/nschema#hasPublicationInfo
pubinfo
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step
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Nanotate
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2021-03-05T10:08:35.231Z
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