http://purl.org/np/RAp_N2Rdw3... RAp_N2Rdw3

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/net/p-plan#isStepOfPlan isStepOfPlan

http://purl.org/np/RAp_N2Rdw3...#plan plan

Nanotate

2021-02-25T16:32:30.020Z

http://purl.org/np/RAp_N2Rdw3... RAp_N2Rdw3

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/np/RAo_IfO6pT...#step step

http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes

Nanotate

2021-02-25T16:32:30.020Z

http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://www.w3.org/1999/02/22-rdf-syntax-ns#type type

http://purl.org/net/p-plan#Step Step

Nanotate

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http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/dc/elements/1.1/description description

After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0).

Nanotate

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http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed

http://purl.org/np/RAqSdY5sD_...#_2 _2

Nanotate

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http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218 C62218

Nanotate

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http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed

http://purl.org/np/RAqSdY5sD_...#_3 _3

Nanotate

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http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed

http://purl.obolibrary.org/obo/CHEBI_16991 CHEBI_16991

Nanotate

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http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed

http://purl.org/np/RAqSdY5sD_...#_1 _1

Nanotate

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http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/net/SMARTprotocol#hasExperimentalOutput hasExperimentalOutput

http://purl.obolibrary.org/obo/CHEBI_16991 CHEBI_16991

Nanotate

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http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo

http://purl.org/np/RAqSdY5sD_... RAqSdY5sD_

http://purl.org/nanopub/x/introduces introduces

Nanotate

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