References for: step

Full identifier: http://purl.org/np/RAZQSfTf7hdqYE1qFLykuQB4o3Oftn9vZTlXwsJpPLiCM#step

Nanopublication Part Subject Predicate Object Published By Published On
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step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:18:38.096Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates.
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-05T10:03:51.750Z
links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo
step
Nanotate
2021-03-05T10:03:51.750Z