http://purl.org/np/RArGkmhKwX... RArGkmhKwX

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/net/p-plan#isStepOfPlan isStepOfPlan

http://purl.org/np/RArGkmhKwX...#plan plan

Nanotate

2021-03-05T10:18:38.096Z

http://purl.org/np/RArGkmhKwX... RArGkmhKwX

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes

http://purl.org/np/RATUKFz980...#step step

Nanotate

2021-03-05T10:18:38.096Z

http://purl.org/np/RArGkmhKwX... RArGkmhKwX

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/np/RAjeakz_ED...#step step

http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes

Nanotate

2021-03-05T10:18:38.096Z

http://purl.org/np/RAZQSfTf7h... RAZQSfTf7h

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://www.w3.org/1999/02/22-rdf-syntax-ns#type type

http://purl.org/net/p-plan#Step Step

Nanotate

2021-03-05T10:03:51.750Z

http://purl.org/np/RAZQSfTf7h... RAZQSfTf7h

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/dc/elements/1.1/description description

Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates.

Nanotate

2021-03-05T10:03:51.750Z

http://purl.org/np/RAZQSfTf7h... RAZQSfTf7h

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed

http://purl.org/np/RAZQSfTf7h...#_3 _3

Nanotate

2021-03-05T10:03:51.750Z

http://purl.org/np/RAZQSfTf7h... RAZQSfTf7h

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed

http://purl.org/np/RAZQSfTf7h...#_2 _2

Nanotate

2021-03-05T10:03:51.750Z

http://purl.org/np/RAZQSfTf7h... RAZQSfTf7h

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/net/SMARTprotocol#hasExperimentalOutput hasExperimentalOutput

http://purl.org/np/RAZQSfTf7h...#_1 _1

Nanotate

2021-03-05T10:03:51.750Z

http://purl.org/np/RAZQSfTf7h... RAZQSfTf7h

links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo

http://purl.org/np/RAZQSfTf7h... RAZQSfTf7h

http://purl.org/nanopub/x/introduces introduces

Nanotate

2021-03-05T10:03:51.750Z