References for: step

Full identifier: http://purl.org/np/RAYDwTtT3SWmPi5y6QPlgud6dumB_mJt6XQ3oYluNNiVU#step

Nanopublication Part Subject Predicate Object Published By Published On
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step
Nanotate
2021-02-24T16:10:21.737Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T16:10:21.737Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
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Nanotate
2021-02-24T16:10:21.737Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it.
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-24T15:57:29.331Z
links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo
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Nanotate
2021-02-24T15:57:29.331Z