http://purl.org/np/RArGkmhKwX... RArGkmhKwX

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/net/p-plan#isStepOfPlan isStepOfPlan

http://purl.org/np/RArGkmhKwX...#plan plan

Nanotate

2021-03-05T10:18:38.096Z

http://purl.org/np/RArGkmhKwX... RArGkmhKwX

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/np/RAZQSfTf7h...#step step

http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes

Nanotate

2021-03-05T10:18:38.096Z

http://purl.org/np/RATUKFz980... RATUKFz980

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://www.w3.org/1999/02/22-rdf-syntax-ns#type type

http://purl.org/net/p-plan#Step Step

Nanotate

2021-03-05T10:02:33.868Z

http://purl.org/np/RATUKFz980... RATUKFz980

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/dc/elements/1.1/description description

Cells can be treated with different antigens for different period of times and the supernatants can be analysed for cytokine levels. The cells can be analysed for phenotypic change, apoptosis or proliferation. Note: PBMCs are primary cells and cannot be cultured for more than one passage under normal conditions. Lymphocytes of PBMCs can be made to proliferate in vitro by mitogens e.g., Phytohaemagglutin or Concanavin-A etc over a period to 72-96 h. Monocytes generally are end cells and do not proliferate. In absence of mitogens the proliferation of PBMCs will be negligible.

Nanotate

2021-03-05T10:02:33.868Z

http://purl.org/np/RATUKFz980... RATUKFz980

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed

http://purl.org/np/RATUKFz980...#_2 _2

Nanotate

2021-03-05T10:02:33.868Z

http://purl.org/np/RATUKFz980... RATUKFz980

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/net/SMARTprotocol#hasExperimentalOutput hasExperimentalOutput

http://purl.org/np/RATUKFz980...#_1 _1

Nanotate

2021-03-05T10:02:33.868Z

http://purl.org/np/RATUKFz980... RATUKFz980

links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo

http://purl.org/np/RATUKFz980... RATUKFz980

http://purl.org/nanopub/x/introduces introduces

Nanotate

2021-03-05T10:02:33.868Z