http://purl.org/np/RAQ8Vn8Nkp... RAQ8Vn8Nkp

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/net/p-plan#isStepOfPlan isStepOfPlan

http://purl.org/np/RAQ8Vn8Nkp...#plan plan

Nanotate

2021-04-21T14:44:11.546Z

http://purl.org/np/RAQ8Vn8Nkp... RAQ8Vn8Nkp

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes

http://purl.org/np/RA0vn8x1MS...#step step

Nanotate

2021-04-21T14:44:11.546Z

http://purl.org/np/RAQ8Vn8Nkp... RAQ8Vn8Nkp

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/np/RAJODcXkxb...#step step

http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes

Nanotate

2021-04-21T14:44:11.546Z

http://purl.org/np/RAMb6wb2si... RAMb6wb2si

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://www.w3.org/1999/02/22-rdf-syntax-ns#type type

http://purl.org/net/p-plan#Step Step

Nanotate

2021-03-10T11:18:25.462Z

http://purl.org/np/RAMb6wb2si... RAMb6wb2si

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

http://purl.org/dc/elements/1.1/description description

Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath. Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.

Nanotate

2021-03-10T11:18:25.462Z

http://purl.org/np/RAMb6wb2si... RAMb6wb2si

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed

http://purl.obolibrary.org/obo/OBI_0400155 OBI_0400155

Nanotate

2021-03-10T11:18:25.462Z

http://purl.org/np/RAMb6wb2si... RAMb6wb2si

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed

http://purl.org/np/RAMb6wb2si...#_2 _2

Nanotate

2021-03-10T11:18:25.462Z

http://purl.org/np/RAMb6wb2si... RAMb6wb2si

links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed

http://purl.org/np/RAMb6wb2si...#_1 _1

Nanotate

2021-03-10T11:18:25.462Z

http://purl.org/np/RAMb6wb2si... RAMb6wb2si

links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo

http://purl.org/np/RAMb6wb2si... RAMb6wb2si

http://purl.org/nanopub/x/introduces introduces

Nanotate

2021-03-10T11:18:25.462Z