References for: step

Full identifier: http://purl.org/np/RA8vn2YWrFMiWvNBVO2inHF2gTHdUjQwkF8PVz4txJLyM#step

Nanopublication Part Subject Predicate Object Published By Published On
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step
Nanotate
2021-04-21T13:37:05.476Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:37:05.476Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:37:05.476Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:40:20.117Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette. Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.
Nanotate
2021-03-20T05:40:20.117Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:40:20.117Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:40:20.117Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:40:20.117Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:40:20.117Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-20T05:40:20.117Z
links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo
step
Nanotate
2021-03-20T05:40:20.117Z