References for: step
Full identifier: http://purl.org/np/RA6F1VxP7thp4zX-1raNBn2QGqYfK0d8XZO_-jUUWiqNE#step
Nanopublication | Part | Subject | Predicate | Object | Published By | Published On |
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
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step
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Nanotate
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2021-03-22T16:05:00.244Z
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
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step
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Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette.
Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.
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Nanotate
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2021-03-22T16:05:00.244Z
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-03-22T16:05:00.244Z
|
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links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-03-22T16:05:00.244Z
|
|||
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-03-22T16:05:00.244Z
|
|||
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-03-22T16:05:00.244Z
|
|||
links a nanopublication to its assertion
http://www.nanopub.org/nschema#hasAssertion
assertion
|
step
|
Nanotate
|
2021-03-22T16:05:00.244Z
|
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links a nanopublication to its pubinfo
http://www.nanopub.org/nschema#hasPublicationInfo
pubinfo
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step
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Nanotate
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2021-03-22T16:05:00.244Z
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