Nanopublication: RAMb6wb2si
Full identifier: http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#_1
_1
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"suspension"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#_2
_2
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"100 mg/ml lysozyme"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#step
step
http://purl.org/dc/elements/1.1/description
description
(this is a literal)
"Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.
Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield."
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#step
step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
type
http://purl.org/net/p-plan#Step
Step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#_1
_1
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
http://purl.obolibrary.org/obo/OBI_0400155
OBI_0400155
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#_2
_2
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAMb6wb2si...#assertion
The assertion above
http://purl.org/pav/createdWith
createdWith
https://nanotate.bitsfetch.com
nanotate.bitsfetch.com
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAMb6wb2si...#assertion
The assertion above
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-03-10T11:18:25.462488"
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAMb6wb2si...#assertion
The assertion above
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/alejandra.sandoval
alejandra.sandoval
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAMb6wb2si...
This nanopublication
the nanopublication consists of an introduction of the given resource
http://purl.org/nanopub/x/introduces
introduces
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#step
step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#sig
sig
http://purl.org/nanopub/x/hasSignatureTarget
has as target
This is the identifier for this whole nanopublication.
http://purl.org/np/RAMb6wb2si...
this nanopublication
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#sig
sig
http://purl.org/nanopub/x/hasAlgorithm
has the algorithm
(this is a literal)
"RSA"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#sig
sig
http://purl.org/nanopub/x/hasPublicKey
has the public key
(this is a literal)
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RAMb6wb2si...#sig
sig
http://purl.org/nanopub/x/hasSignature
has the signature
(this is a literal)
"AVq49EuW85NpvUlB6CB8eVMe0Jr5EYEXDCQYm9p6fF1znfEISpbUsiRzXIgCwVWYoRJ3zshHUNexjRblahiYJOvvQnwongr8MNHjhUhFhqJjgXG8trQhZ2uL0DwPB3OzOSuI9rz03wCdTzRcSrr9OSHxoqY81B5G6SBcQpeGSG8="
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAMb6wb2si...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/EF-yuIFSEeuet6vjBV_k6Q
EF-yuIFSEeuet6vjBV_k6Q
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAMb6wb2si...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/JfaRoIFSEeu374uFEHtqAg
JfaRoIFSEeu374uFEHtqAg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAMb6wb2si...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/K0yIKoG8EeujNmf67OBRmw
K0yIKoG8EeujNmf67OBRmw
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RAMb6wb2si...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/tmG8cIFREeuamj8DU21Bpw
tmG8cIFREeuamj8DU21Bpw
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAMb6wb2si...
this nanopublication
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-03-10T11:18:25.462488"
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RAMb6wb2si...
this nanopublication
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/alejandra.sandoval
alejandra.sandoval
.