Nanopublication: RA8LXJxDVi
Full identifier: http://purl.org/np/RA8LXJxDVioLwvnT7U0GREq_szXgcjzp3WYAyInmPlWNQ
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#_1
_1
http://www.w3.org/2000/01/rdf-schema#label
label
(this is a literal)
"lysozyme solution"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#step
step
http://purl.org/dc/elements/1.1/description
description
(this is a literal)
"Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.
Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield."
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#step
step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
type
http://purl.org/net/p-plan#Step
Step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#step
step
https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed
bioSampleUsed
http://www.bioassayontology.org/bao#BAO_0002104
BAO_0002104
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#step
step
https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed
labEquipmentUsed
http://purl.obolibrary.org/obo/OBI_0400155
OBI_0400155
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#step
step
https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed
reagentUsed
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#_1
_1
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA8LXJxDVi...#assertion
The assertion above
http://purl.org/pav/createdWith
createdWith
https://nanotate.bitsfetch.com
nanotate.bitsfetch.com
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA8LXJxDVi...#assertion
The assertion above
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-02-26T09:44:15.524571"
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA8LXJxDVi...#assertion
The assertion above
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
zuluaga.lizzz
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RA8LXJxDVi...
This nanopublication
the nanopublication consists of an introduction of the given resource
http://purl.org/nanopub/x/introduces
introduces
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#step
step
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#sig
sig
http://purl.org/nanopub/x/hasSignatureTarget
has as target
This is the identifier for this whole nanopublication.
http://purl.org/np/RA8LXJxDVi...
this nanopublication
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#sig
sig
http://purl.org/nanopub/x/hasAlgorithm
has the algorithm
(this is a literal)
"RSA"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#sig
sig
http://purl.org/nanopub/x/hasPublicKey
has the public key
(this is a literal)
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
.
This is a local identifier minted within the nanopublication.
http://purl.org/np/RA8LXJxDVi...#sig
sig
http://purl.org/nanopub/x/hasSignature
has the signature
(this is a literal)
"fHtQyp6CR1dzknNYSulii53Kpdx63g3ELHhARErrwVgcSxWDhvAeQhEPO6hAGppZitcTqNsyFZd9SXgrBy11gVFRB7ghF97zELzr5YgycWfkPd+ggsnjOS/EvFa/Dyx3R6eRdNOV36npp0akvuhqvFSDIer7hkd5VxmNTm08B7k="
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA8LXJxDVi...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/Mm4SaHeyEeupA9_TrHGoQQ
Mm4SaHeyEeupA9_TrHGoQQ
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA8LXJxDVi...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/SxYACneyEeuhZweY7act7g
SxYACneyEeuhZweY7act7g
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA8LXJxDVi...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/f0Q7MHeyEeum_5cx2E1wjg
f0Q7MHeyEeum_5cx2E1wjg
.
This is the identifier for the assertion of this nanopublication.
http://purl.org/np/RA8LXJxDVi...#assertion
the assertion above
http://www.w3.org/ns/prov#wasDerivedFrom
wasDerivedFrom
https://hypothes.is/a/osRxYHeyEeu5zVuedDPw0Q
osRxYHeyEeu5zVuedDPw0Q
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RA8LXJxDVi...
this nanopublication
http://www.w3.org/ns/prov#generatedAtTime
generatedAtTime
(this is a literal)
"2021-02-26T09:44:15.524571"
.
This is the identifier for this whole nanopublication.
http://purl.org/np/RA8LXJxDVi...
this nanopublication
http://www.w3.org/ns/prov#wasAttributedTo
wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
zuluaga.lizzz
.