step

Full identifier: http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#step

Assigned to 1 class:

Minted in Nanopublication

 RAeY0Vlxxi Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "pellet" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "tubes" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#_3 _3 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "TE" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#step step https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#_1 _1 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#step step https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#_2 _2 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#_3 _3 .
This is the identifier for this whole nanopublication. http://purl.org/np/RAeY0Vlxxi... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAeY0Vlxxi...#step step .

References

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:24:12.850Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T13:24:12.850Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-11T16:39:49.461Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0).
Nanotate
2021-03-11T16:39:49.461Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-11T16:39:49.461Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-11T16:39:49.461Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-11T16:39:49.461Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-11T16:39:49.461Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-11T16:39:49.461Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-11T16:39:49.461Z

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