step

Full identifier: http://purl.org/np/RAi9SKo7-re6vO3nWPJzZcthuREF-Hjsi2hlwJk9OmRUE#step

Assigned to 1 class:

Minted in Nanopublication

 RAi9SKo7-r Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "recalcitrant samples" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "recalcitrant samples" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_3 _3 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mortar" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_4 _4 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "pestle" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_5 _5 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "liquid nitrogen" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_6 _6 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "extraction buffer" .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#step step http://purl.org/net/SMARTprotocol#hasExperimentalInput hasExperimentalInput This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_1 _1 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#step step https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_2 _2 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#step step https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_3 _3 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#step step https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_4 _4 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_5 _5 .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#_6 _6 .
This is the identifier for this whole nanopublication. http://purl.org/np/RAi9SKo7-r... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#step step .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAi9SKo7-r... this nanopublication .
This is a local identifier minted within the nanopublication. http://purl.org/np/RAi9SKo7-r...#sig sig http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal) "RSA" .

Nanotate, 10 Mar 2021, 10:16:14 UTC

References

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T14:22:28.097Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-04-21T14:22:28.097Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
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Nanotate
2021-03-10T10:16:14.459Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples
Nanotate
2021-03-10T10:16:14.459Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T10:16:14.459Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T10:16:14.459Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T10:16:14.459Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T10:16:14.459Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T10:16:14.459Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-03-10T10:16:14.459Z

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